Electroduction on Azospirillum brasilense Sp7 and Chromobacterium violaceum
DOI:
https://doi.org/10.54987/jobimb.v8i2.537Keywords:
Electroduction; Azospirillum brasilense Sp7; Chromobacterium violaceum; cosmid vector pLAFR1Abstract
Electroduction is a fast, simple and very efficient transformation method with a wider range of bacteria strains which involved physical process by exposing to high electricity. A successful transfer of a 21.6 kb cosmid vector pLAFR1 into Azospirillum brasilense Sp7 and Chromobacterium violaceum was achieved with the maximum efficiency of about 35 X 102 and 24 X 102 transformants, respectively, at the electrical field strength of 5kV/cm with longer pulse length range between 8.3 and 8.5 milliseconds. Doubling the pulse length and a decrease or increase in the electrical field strength of 5kV/cm resulted in a reduction in the total number of transformants. The electroduction parameters were very important to make this transformation method works efficiently.
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