Expression, purification and characterization of meta-cleavage enzyme CarBaBb from Novosphiongobium sp. KA1

Authors

  • Azham Zulkharnain Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
  • R. Maeda Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
  • T. Maeda Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

DOI:

https://doi.org/10.54987/jobimb.v1i1.378

Keywords:

meta=cleavage enzyme, extradiol dioxygenase, carbazole, degradation

Abstract

The meta-cleavage enzyme CarBaBb of carbazole-degrader Novosphiongobium sp. KA1 were cloned, expressed and purified to homogeneity in Escherichia coli strain. The enzyme was cloned with 6x histidine residues attached at the C-terminal of large subunit CarBb for purification using affinity chromatography method prior to gel filtration chromatography. The CarBaBb, a two-subunit meta-cleavage enzyme, approximately 30 kda for CarBb dan 10 kDa for CarBa, was found to be α2β2-heterotetrameric (Mr 80,000), showed highest activity at pH 8.5 and temperature 30°C. CarBaBb showed highest catalytic activity towards 2,3-dihydroxybiphenyl with kcat/Km 4.1 M-1s-1, and overall higher catalytic activities towards biphenyl-type substrates in comparison to catechol-type substrates. Based on the similarities, this meta-cleavage enzyme from Novosphiongobium sp. KA1 would also be a good candidate for protein crystallization and structural studies apart from CarBaBb from strain P. resinovorans strain CA10.

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Published

31.12.2013

Issue

Vol. 1 No. 1 (2013)

Section

Articles

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How to Cite

Expression, purification and characterization of meta-cleavage enzyme CarBaBb from Novosphiongobium sp. KA1. (2013). Journal of Biochemistry, Microbiology and Biotechnology, 1(1), 11-16. https://doi.org/10.54987/jobimb.v1i1.378