Integrated Factors Influencing Bacterial Cellulose Production in Kombucha SCOBY: Bioprocess Strategies for Enhanced Yield
DOI:
https://doi.org/10.54987/jobimb.v13i1.1078Keywords:
Bacterial cellulose, Kombucha, Bioprocess, Fermentation, ReviewAbstract
Bacterial cellulose (BC) is an eco-friendly biopolymer with unique properties, including high purity, mechanical strength, and biocompatibility. Its production is influenced by fermentation conditions such as carbon sources (e.g., sucrose, glucose), nutrient composition, temperature (25-32 °C), pH (4.0-7.5), and aeration. Static cultivation yields highly crystalline BC with layered structures, while agitated systems enhance productivity but reduce mechanical integrity. Process optimization using waste-derived substrates (e.g., molasses, fruit residues) and statistical modeling (e.g., RSM) improves cost efficiency and sustainability. BC's nanofibrillar structure provides exceptional tensile strength (200-300 MPa), high water retention (up to 98%), and thermal stability (decomposition at 300-350 °C). These properties make it valuable in biomedical applications (wound dressings, tissue engineering), food packaging (edible films), and industrial uses (nanocomposites, filtration membranes). However, scaling up production faces challenges, including genetic instability in continuous cultures, shear stress in bioreactors, and high downstream processing costs. Recent advancements focus on metabolic engineering, hybrid fermentation systems, and immobilized cell techniques to enhance yield and scalability. BC's potential as a sustainable alternative to synthetic materials is promising, particularly in medicine and green manufacturing. However, overcoming production cost and yield limitations remains critical for broader industrial adoption. Future research should optimize strain-specific fermentation, integrate circular bioeconomy principles, and refine functionalization techniques to expand BC’s commercial applications.
References
Feng X, Ge Z, Wang Y, Xia X, Zhao B, Dong M. Production and characterization of bacterial cellulose from kombucha-fermented soy whey. Food Prod Process Nutr. 2024; 6(20). doi:10.1186/s43014-023-00188-3
Vukmanovi? S, Vitas J, Ranitovi? A, Cvetkovi? DD, Tomi? A, Malbaša RV. Certain production variables and antimicrobial activity of novel winery effluent based kombucha. LWT - Food Sci Technol. 2022; 154, 112726. doi:10.1016/J.LWT.2021.112726
Cubas ALV, Gouveia IC, Mouro C, Provin AP, Dutra ARA. Advances in the production of biomaterials through kombucha using food waste: concepts, challenges, and potential. Polymers. 2023; 15(7), 1701. doi:10.3390/polym15071701
Su J, Tan Q, Tang Q, Tong Z, Yang M. Research progress on alternative kombucha substrate transformation and the resulting active components. Front Microbiol. 2023; 14. doi:10.3389/fmicb.2023.1254014
Pihurov M, Borda D, Grigore-Gurgu L, Kluz M, Bahrim GE, P?cularu-Burada B, Cotârle? M, St?nciuc N. Kombucha and water kefir grains microbiomes' symbiotic contribution to postbiotics enhancement. Foods. 2023; 12(13), 2581. doi:10.3390/foods12132581
Srivastava S, Mathur G. Statistical optimization of bioprocess parameters for enhanced production of bacterial cellulose from K. saccharivorans BC-G1. Brazilian J. Microb. 2024; 55:2199-2210. doi:10.1007/s42770-024-01397-9
Selvaraj A, Gurumurthy P. Optimizing bacterial cellulose production from kombucha tea. Ferment Sci Technol. 2024; 12(1), 45-58. doi: 10.1016/j.fst.2023.102345
Aditiawati P, Dungani R, Muharam S, Sulaeman A, Hartati S, Dewi M, Rosamah E. The nanocellulose fibers from symbiotic culture of bacteria and yeast (SCOBY) kombucha: preparation and characterization. Nanofibers-Synthesis, Properties and Applications. IntechOpen 2021. doi:10.5772/intechopen.96310
Wang SS, Shi XX, Chen DL, Ye YX, Chen JL, Li M, Zhang DC, Han YH. Insights into bacterial cellulose biosynthesis from different carbon sources and the associated biochemical transformation pathways in Komagataeibacter sp. W1. Polymers. 2018; 10(9):963. doi:10.3390/polym10090963
Molina-Ramírez C, Rojas O, Gómez C, Zuluaga R, Castro C, Osorio M, Torres-Taborda M, Gañán P, Gómez B, Castro M. Effect of different carbon sources on bacterial nanocellulose production and structure using the low pH resistant strain Komagataeibacter Medellinensis. Materials. 2017; 10(6), 639. doi:10.3390/ma10060639
Krystynowicz A, Czaja W, Wiktorowska-Jezierska A, Gonçalves-Mi?kiewicz M, Turkiewicz M. Bielecki S. Factors affecting the yield and properties of bacterial cellulose. J Ind Microbiol Biotechnol. 2002; 29(4): 189-195. doi:10.1038/sj.jim.7000303
Lahiri D, Nag M, Dutta B, Dey A, Sarkar T, Pati S, Edinur HA, Abdul Kari Z, Mohd Noor NH, Ray RR. Factors affecting bacterial cellulose biosynthesis and its biomedical applications. Int J Mol Sci. 2021; 22(11): 12984. doi:10.3390/ijms22112984
U?urel C, Ö?üt H. Optimization of bacterial cellulose production by Komagataeibacter rhaeticus K23. Fibers. 2024; 12(3), 29. doi:10.3390/fib12030029
Lee KY, Bismarck A, Buldum G, Mantalaris A. More than meets the eye in bacterial cellulose: biosynthesis, bioprocessing, and applications in advanced fiber composites. Macromol Biosci. 2013; 14(1), 10-32. doi:10.1002/mabi.201300298
Jahan F, Kumar V, Rawat G, Saxena RK. Production of microbial cellulose by a bacterium isolated from fruit. Appl Biochem Biotechnol. 2012; 167(5), 1157-71. doi:10.1007/s12010-012-9595-x
El-Gendi H, Taha HT, Ray JB. Saleh AK. Recent advances in bacterial cellulose: a low-cost effective polymer. Cellulose. 2022; 29(5), 7495-7533. doi:10.1007/s10570-022-04697-1
Rahman SSA, Vidyasri GS, Vaishnavi T, Venkatachalam P, Karuppiah S, Sathya K, Priyanka P. Production of bacterial cellulose using Gluconacetobacter kombuchae immobilized on luffa aegyptiaca support. Sci Rep. 2021; 11(1). doi:10.1038/s41598-021-82596-4
Santoso SP, Lin SP, Cheng KC, Ismadji S, Soetaredjo FE, Hsieh CW, Chou CC. Enhanced production of bacterial cellulose by Komactobacter intermedius using statistical modeling. Cellulose. 2020; 27(5), 2497-2509. doi:10.1007/s10570-019-02961-5
Basu A, Lim S, Vadanan SV. A Novel platform for evaluating the environmental impacts on bacterial cellulose production. Sci Rep. 2018; 8(1). doi:10.1038/s41598-018-23701-y
Swingler S, Gupta A, Gibson H, Kowalczuk M, Heaselgrave W, Radecka I. Recent advances and applications of bacterial cellulose in biomedicine. Polymers. 2021; 13(3), 412. doi:10.3390/polym13030412
Gorgieva S, Tr?ek J. Bacterial cellulose: production, modification and perspectives in biomedical applications. Nanomaterials. 2019; 9(10), 1352. doi:10.3390/nano9101352
Panaitescu DM, Frone AN, Chiulan I, Casarica A, Nicolae CA, Ghiurea M, Trusca R, Damian CM. Structural and morphological characterization of bacterial cellulose nano-reinforcements prepared by mechanical route. Mater Des. 2016; 110, 790-801. doi:10.1016/j.matdes.2016.08.052
Ruan C, Zhu Y, Zhou X, Abidi N, Hu Y, Catchmark JM. Effect of cellulose crystallinity on bacterial cellulose assembly. Cellulose. 2016; 23:245-256. doi:10.1007/s10570-016-1065-0
Kosseva MR, Zhong S, Li M, Zhang J, Tjutju NAS. Biopolymers produced from food wastes: A case study on biosynthesis of bacterial cellulose from fruit juices. In biopolymers from food wastes. Elsevier: Amsterdam; 2020. doi.org/10.1016/B978-0-12-817121-9.00011-5
Portela R, Leal CR, Almeida PL, Sobral RG. Bacterial cellulose: A versatile biopolymer for wound dressing applications. Microb. Biotechnol. 2019; 12(4), 586-610. doi:10.1111/1751-7915.13392
Rosson L, Tan B, Best W, Byrne N. Applications of regenerated bacterial cellulose: a review. Cellulose. 2024; 31:10165-10190. doi:10.1007/s10570-024-06220-0
Laavanya D, Shirkole S, Balasubramanian P. Current challenges, applications, and future perspectives of SCOBY cellulose of kombucha fermentation. J Clea Prod. 2021; 295:126454. doi:10.1016/j.jclepro.2021.126454
Cahyaningtyas HAA, Renaldi G, Fibriana F, Mulyani WE. Cost-effective production of kombucha bacterial cellulose by evaluating nutrient sources, quality assessment, and dyeing methods. Environ Sci Pollut Res Int. 2025; 32(5):2713-25. doi:10.1007/s11356-025-35915-5.
Azeredo HMC, Barud H, Farinas CS, Vasconcellos VM, Claro AM. Bacterial cellulose as a raw material for food and food packaging applications. Front Sustain Food Syst. 2019. doi:10.3389/fsufs.2019.00007
Santos MRD, Durval IJB, Medeiros ADM, Silva Júnior CJGD, Converti A, Costa AFS, Sarubbo LA. Biotechnology in food packaging using bacterial cellulose. Foods. 2024; 13(20):3327. doi:10.3390/foods13203327.
Kuci?ska-Lipka J, Janik H, Gubanska I. Bacterial cellulose in the field of wound healing and regenerative medicine of skin: recent trends and future prospectives. Polym Bull. 2015; 72(9):2399-2419. doi:10.1007/s00289-015-1407-3
Liang S. Advances in drug delivery applications of modified bacterial cellulose-based materials. Front Bioeng Biotech. 2023; 11. doi:10.3389/fbioe.2023.1252706
Popa L, Ghica MV, Ionescu DG, Tudoroiu EE, Dinu-Pîrvu CE. Bacterial cellulose-a remarkable polymer as a source for biomaterials tailoring. Materials. 2022; 15(3): 1054. doi:10.3390/ma15031054
Kamal T, Ul-Islam M, Fatima A, Ullah MW, Manan S. cost-effective synthesis of bacterial cellulose and its applications in the food and environmental sectors. Gels. 2022; 8(9): 552. doi:10.3390/gels8090552
Rathinamoorthy R, Kiruba T. Bacterial cellulose-a potential material for sustainable eco-friendly fashion products. J Natural Fibers. 2020; 19(9):3275-3287. doi:10.1080/15440478.2020.1842841
Betlej I, Boruszewski P, Zakaria S, Krajewski KJ. Bacterial cellulose - properties and its potential application. Sains Malaysiana. 2021; 50(2):493-505. doi:10.17576/jsm-2021-5002-20
Mehrotra R, Sharma S, Kaur K, Shree N. Bacterial cellulose: an ecological alternative as a biotextile. Biosci Biot Res Asia. 2023; 20(2):449-463. doi:10.13005/bbra/3101
Klemm?D,?et?al. Cellulose: fascinating biopolymer and sustainable raw material. Angew?Chem?Int?Ed?Engl.?2005;44(22):3358-3393.
Moon RJ, et al. Cellulose nanomaterials review: structure, properties and nanocomposites. Chem?Soc?Rev.?2011;40(7):3941-3994.
Habibi?Y,?et?al. Cellulose nanocrystals: chemistry, self?assembly, and applications. Chem?Rev.?2010;110(6):3479-3500.
Lin?N, Dufresne?A. Nanocellulose in biomedicine: current status and future prospect. Eur?Polym?J.?2014;59:302-325.
Thomas S, et al. Biofibres and biocomposites. Carbohydr?Polym.?2009;77(3):672-674.
Rey?M?W,?et?al. Acetic acid bacteria. Encyclopedia of Life Sciences (ELS).?2003;1-9.
Mamlouk?D, Gullo?M. Acetic acid bacteria: physiology and carbon sources oxidation. Int?J?Food?Microbiol.?2013;162(1):1-14.
Cui Y, Wang D, Zhang L, Qu X. Research progress on the regulatory mechanism of biofilm formation in probiotic lactic acid bacteria. Crit Rev Food Sci Nutr. 2024 Sep 3:1-5.
Flemming?HC, Wingender?J. The biofilm matrix. Nat?Rev?Microbiol.?2010;8(9):623-633.
Gatenholm P, Klemm D. Bacterial nanocellulose as a renewable material for biomedical applications. MRS bulletin. 2010 Mar;35(3):208-13.
Azubuike CC, Chikere CB, Okpokwasili GC. Bioremediation: An eco-friendly sustainable technology for environmental management. In Bioremediation of Industrial Waste for Environmental Safety: Volume I: Industrial Waste and Its Management 2019 Jun 30 (pp. 19-39). Singapore: Springer Singapore.
Carreira P, Mendes JA, Trovatti E, Serafim LS, Freire CS, Silvestre AJ, Neto CP. Utilization of residues from agro-forest industries in the production of high value bacterial cellulose. Bioresour?Technol.?2011;102(15):7354-7360.
Chen HC, Tze WT, Chang FC. Effects of nanocellulose formulation on physicomechanical properties of Aquazol-nanocellulose composites. Cellulose. 2020 Jul;27(10):5757-69.
Jorfi M, Foster EJ. Recent advances in nanocellulose for biomedical applications. J?Appl?Polym?Sci.?2015;132(14):41719.
Deppenmeier U, Ehrenreich A. Physiology of acetic acid bacteria in light of the genome sequence of Gluconobacter oxydans. J Mol Microbiol Biotechnol. 2009;16(1-2):69-80.
Axelsson L. Lactic acid bacteria: classification and physiology. Food Science and Technology-New York-Marcel Dekker. 2004 Jul 23;139:1-66.
Sheltami RM, Kargarzadeh H, Abdullah I, Ahmad I. Thermal properties of cellulose nanocomposites. Handbook of nanocellulose and cellulose nanocomposites. 2017 May 1;2:523-52
Czaja WK, Young DJ, Kawecki M, Brown RM. The future prospects of microbial cellulose in biomedical applications. Biomacromolecules. 2007;8(1):1-12.
Dufresne A. Nanocellulose: from nature to high performance tailored materials. Walter de Gruyter GmbH & Co KG; 2017 Nov 20.
Chawla PR, Bajaj IB, Survase SA, Singhal RS. Microbial cellulose: fermentative production and applications. Food Technol Biotechnol. 2009;47(2):107-124.
Krystynowicz A, Czaja W, Wiktorowska-Jezierska A, Gonçalves-Mi?kiewicz M, Turkiewicz M, Bielecki S. Factors affecting the yield and properties of bacterial cellulose. J Ind Microbiol Biotechnol. 2002;29(4):189-195.
Keshk SM. Bacterial cellulose production and its industrial applications. J Bioprocess Biotech. 2014 Jan 1;4(2):150.
Siró I, Plackett D. Microfibrillated cellulose and new nanocomposite materials: a review. Cellulose. 2010 Jun;17(3):459-94.
Dufresne A. Nanocellulose processing properties and potential applications. Curr For Rep. 2019 Jun 15;5(2):76-89.
Aulesa C, Góngora C. Assessing kombucha: a systematic review of health effects in human. J CAM Res Progress. 2024;2(1):115.
Villarreal-Soto SA, Beaufort S, Bouajila J, Souchard JP, Renard T, Rollan S, Taillandier P. Impact of fermentation conditions on the production of bioactive compounds with anticancer, anti-inflammatory and antioxidant properties in kombucha tea extracts. Process Biochem. 2019 Aug 1;83:44-54.
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