Evaluation of Phytochemical Components and Antidiarrheal Activity of Hydro-Methanolic Extracts of Carica papaya Seeds in Castor Oil-Induced Diarrhea in Wistar Rats
DOI:
https://doi.org/10.54987/jemat.v12i2.1059Keywords:
Carica papaya seed, Wistar rats, Antidiarrheal, Laperomide, AntibioticAbstract
Carica papaya, popularly known as pawpaw in English, is a plant that grows worldwide and bears leaves, fruits, and seeds with many medicinal properties, including anti-inflammatory, antidiarrheal, and antibiotic effects. The seeds of Carica papaya were collected at Dandagoro Garden and authenticated by a botanist at Ummaru Musa Yar'adua University, Katsina, with voucher number UMYUK/P/105.This study aimed to evaluate the antidiarrheal activity of the hydro-methanolic extract of Carica papaya seeds in Wistar rats using a negative and positive control. The seed extract was administered to Wistar rats (weighing 162 g) at different doses in each of the five groups, each containing five Wistar rats. Thirty minutes after the administration of the extracts, diarrhea was induced using castor oil. The quantitative determination of phytochemical constituents in Carica papaya seeds was performed using a UV spectrophotometric method. The results shows that alkaloids having the highest amount of (24.03±0.08 mg/100 g) whereas tannins having the lesser amount (0.02±0.01 mg/100 g), also the antioxidant activities of Carica papaya seed extract and its effects on some biomarkers where determined which includes the Intestinal Protein (IP), malondialdehyde (MDA), reduced glutathione(GSH), superoxide dismutase(SOD), alkaline phosphatase (AP), nitric oxide (NO) and Na+-K+ ATPase. The levels of IP, SOD and AP shows a significant decrease when compared to the negative control at (p≤0.05). The levels of NO, GSH, and Na+-K+ ATPase show a significant increase compared to the negative control (p ≤ 0.05). The levels of MDA do not show any significant increase or decrease when compared to the negative control (p ≥ 0.05). In conclusion, the findings of this study show that Carica papaya seeds have antidiarrheal properties, albeit in a lesser amount, which may be due to their lower flavonoid content. However, Carica papaya seeds can be used to control diarrhea before an absolute proper medication.
References
Wink M. Modes of action of herbal medicines and plant secondary metabolites. Medicines (Basel). 2015;2(3):251-286.
Guerrant RL, Van Gilder T, Steiner TS, Thielman NM, Slutsker L, Tauxe RV. Practice guidelines for the management of infectious diarrhea. Clin Infect Dis. 2001;32(3):331-335.
Mehmood MH, Siddiqi HS, Gilani AH. The antidiarrheal and spasmolytic activities of Phyllanthus emblica are mediated through dual blockade of muscarinic receptors and Ca²? channels. J Ethnopharmacol. 2011;133(2):856-865.
Boschi-Pinto C, Velebit L, Shibuya K. Estimating child mortality due to diarrhoea in developing countries. Bull World Health Organ. 2008;86(9):710-717.
Hamer DH, Simon J, Thea D, Keusch GT. Childhood diarrhea in Sub-Saharan Africa. Child Health Res Proj Spec Rep. 1998;1(3):1-40.
Uddin S, Chowdhury V, Uddin SB, Howlader MSA. Ethnomedicinal plants used for the treatment of diarrhoea and dysentery by the Lushai community in Bandarban district, Bangladesh. J Adv Med Life Sci. 2015;2(14). doi:10.15297/jals.V214.05
Abba K, Sinfield R, Hart CA, Garner P. Pathogens associated with persistent diarrhoea in children in low- and middle-income countries: systematic review. BMC Infect Dis. 2009;9:88.
Mishra A, Seth A, Maurya SK. Therapeutic significance and pharmacological activities of antidiarrheal medicinal plants mentioned in Ayurveda: a review. J Intercult Ethnopharmacol. 2016;5(3):290-307.
Thapar N, Sanderson IR. Diarrhoea in children: an interface between developing and developed countries. Lancet. 2004;363(9409):641-653.
World Health Organization. The world health report 2004. Geneva: WHO; 2004. p. 120-125.
Ezekwesili CN, Obiora KA, Ugwu OP. Evaluation of anti-diarrhoeal property of crude aqueous extract of Ocimum gratissimum L. (Labiatae) in rats. Biokemistri. 2004;16(2):122-131.
UNICEF. Pneumonia and diarrhoea: tackling the deadliest diseases for the world's poorest children. New York: United Nations Children's Fund; 2012.
Pandey C, Walpole PJ, Cabot PN, Shaw J, Batra AK, Hewavitharana AK. Selective antiproliferative activities of Carica papaya leaf juice extracts against prostate cancer. Biomed Pharmacother. 2017;89:515-523.
Owoyele BV, Oladosu AA. Carica papaya (Pawpaw) unripe fruit may be beneficial in the prevention and control of hypertension. Indian J Pharmacol. 2010;42(6):392.
Jaiswal S, Mehta S, Naik PK. Papaya (Carica papaya L.): an overview. Int J Res Pharm Chem. 2012;2(1):68-77.
Lutterodt GD. Inhibition of microlax-induced experimental diarrhoea with narcotic-like extracts of Psidium guajava leaf in rats. J Ethnopharmacol. 1992;37(2):151-157.
Owoyele BV, Oladosu AA. Carica papaya (pawpaw) unripe fruit may be beneficial in the prevention and control of hypertension. Indian J Pharmacol. 2010;42(6):392.
Jaiswal S, Mehta S, Naik PK. Papaya (Carica papaya L.): an overview. Int J Res Pharm Chem. 2012;2(1):4-16.
Guerrant RL, Van Gilder T, Steiner TS, Thielman NM, Slutsker L, Tauxe RV. Practice guidelines for the management of infectious diarrhea. Clin Infect Dis. 2001;32(3):331-351.
Lutterodt GD. Inhibition of microlax-induced experimental diarrhoea with narcotic-like extracts of Psidium guajava leaf in rats. J Ethnopharmacol. 1992;37(2):151-157.
Kouitcheu M, Penlap B, Kouam J, Ngadjui BN, Fomum Z, Etoa FX. Evaluation of antidiarrhoeal activity of the stem bark of Cylocodiscus gabunensis (Mimosaceae). Afr J Biotechnol. 2006;5(11):1062-1066.
Knecht H, Neulinger SC, Heinsen FA, et al. Effects of ?-lactam antibiotics and fluoroquinolones on human gut microbiota in relation to Clostridium difficile-associated diarrhea. PLoS One. 2014;9(2):e89417.
Maïkere-Faniyo R, Van Puyvelde L, Mutwewingabo A, Habiyaremye FX. Study of Rwandese medicinal plants used in the treatment of diarrhoea I. J Ethnopharmacol. 1989;26(2):101-109.
Ezekwesili CN, Obiora KA, Ugwu OP. Evaluation of anti-diarrhoeal property of crude aqueous extract of Ocimum gratissimum L. (Labiatae) in rats. Biokemistri. 2004;16(2):122-131.
Havagiray R, Ramesh C, Sadhna K. Study of antidiarrhoeal activity of Calotropis gigantea R.Br. in experimental animals. J Pharm Pharm Sci. 2004;7(1):70-75.
World Health Organization. Diarrhoeal disease: Fact sheet N°330. Geneva: WHO; 2009.
Jones P, Kinghorn D. Extraction of plant secondary metabolites. In: Sarker SD, Latif Z, Gray AI, editors. Natural Products Isolation. 2nd ed. Totowa, NJ: Humana Press; 2006. p. 269-273.
Lawal KR. Practical Pharmacognosy: Techniques and Experiments. 2nd ed. Pune: Nirali Prakashan; 2000. p. 149-155.
Ayoola PB, Adeyeye A. Phytochemical and nutrient evaluation of Carica papaya (pawpaw) leaves. Int J Res Rev Appl Sci. 2010;5(3):325-328.
Baiyewu RA, Amusa NA. Effect of temperature and relative humidity on pawpaw fruit rot in South-Western Nigeria. World J Agric Sci. 2005;1(1):80-83.
Wondmagegn TT, Abebe EH, Abyot EG, Abraham FM. Experimental assessment of anti- and antisecretory activity of 80% methanolic leaf extract of Zehneria scabra in mice. BMC Complement Altern Med. 2014;14:460.
Atta AH, Mouneir SM. Evaluation of some medicinal plant extracts for antidiarrhoeal activity. Phytother Res. 2005;19(6):481-485.
Agbor GA, Leopold T, Jeanne NY. The antidiarrhoeal activity of Alchornea cordifolia leaf extract. Phytother Res. 2004;18(11):873-876.
Umer S, Tekewe A, Kebede N. Antidiarrhoeal and antimicrobial activity of Calpurnia aurea leaf extract. BMC Complement Altern Med. 2013;13:21.
Akhlaq A, Mehmood MH, Rehman A, et al. The prokinetic, laxative, and antidiarrheal effects of Morus nigra: possible muscarinic, Ca²? channel blocking, and antimuscarinic mechanisms. Phytother Res. 2016;30(8):1362-1376.
Vieira C, Evangelista S, Cirillo R, Lippi A, Maggi CA, Manzini S. Effect of ricinoleic acid in acute and subchronic experimental models of inflammation. Mediators Inflamm. 2000;9(5):223-228.
Capasso F, Mascolo N, Izzo AA, Gaginella TS. Dissociation of Castor oil-induced diarrhoea and intestinal mucosal injury in rat: effect of N(G)-nitro-L-arginine methyl ester. Br J Pharmacol. 1994;113(4):1127-1130.
Imam MZ, Sultana S, Akter S. Antinociceptive, antidiarrheal, and neuropharmacological activities of Barringtonia acutangula. Pharm Biol. 2012;50(9):1078-1084.
Deore SR, Namdeo AG. In vitro evaluation of antibacterial activity of Cordia dichotoma Forst on urinary tract pathogens. J Pharm BioSci. 2013;3:110-113.
Sakkir S, Kabshawi M, Mehairbi M. Medicinal plant diversity and their conservation status in the United Arab Emirates (UAE). J Med Plants Res. 2012;6(7):1304-1322.
Zanna M, Shettima AY, Daja A. Antidiarrhoeal effects of aqueous leaf extract of Carica papaya in Wistar strain albino rats. J Sci Multidiscip Res. 2017;9(2):1-6.
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