Isolation, Identification and Antibacterial Activity of Lactic Acid Bacteria Isolated From Traditionally Fermented Raw Milk
DOI:
https://doi.org/10.54987/jebat.v7i2.1048Keywords:
Lactic acid bacteria, Antibacterial activity, Traditionally fermented milk, Escherichia coli, Staphylococcus aureusAbstract
Lactic acid bacteria are well known for their antibacterial activity against pathogenic and spoilage organisms. This study was conducted to determine the antibacterial activity of lactic acid bacteria isolated from traditionally fermented raw milk on E.coli and S. aureus. Three (3) Lactic acid bacteria species were isolated from traditionally fermented raw milk samples collected from different areas within Gombe metropolis. The isolates were identified using cultural, microscopic and biochemical test. The antibacterial activity of the lactic acid bacteria species against E. coli and S. aureus was determined using overlay method. All the different isolates showed growth inhibitory activity against E. coli and S. aureus with sample from Herwagana having the highest average growth inhibitory activity of 17.5 mm on E. coli. The Lactic acid bacteria isolates from traditionally fermented raw milk were shown to have activity on bacterial pathogens. The bacteriocin produced by LAB might be used as the valid candidates of preservative against foodborne pathogens.
References
Hayek SA, Ibrahim SA. Current limitations and challenges with lactic acid bacteria: A review. Food Nutr Sci. 2013;4(1):73-87.
Aspri M, Bozoudi D, Tsaltas D, Hill C, Papademas P. Raw donkey milk as a source of Enterococcus diversity: Assessment of their technological properties and safety characteristics. Food Control. 2017;73:81-90.
Tulini FL, Hymery N, Haertle T, Blay GL, Martinus DE. Screening for antimicrobial and proteolytic activities of lactic acid bacteria isolated from cow, buffalo and goat milk and cheeses marketed in the southeast region of Brazil. J Dairy Res. 2016;83(1):115-24.
Perin LM, Belviso S, Bello B, Dal Nero LA, Cocolin L. Technological properties and biogenic amines production by bacteriocinogenic Lactococci and Enterococci strains isolated from raw goat's milk. J Food Prot. 2017;80(1):151-8.
Deegan LH, Cotter PD, Hill C, Ross P. Bacteriocins: Biological tools for bio-preservation and shelf-life extension. Int Dairy J. 2006;16(9):1058-71.
Makut M, Emelogu N, Ekeleme I, Owuna J, Alfa F. Antimicrobial activity of lactic acid bacteria isolated from locally fermented cow milk products sold in Keffi, Nigeria on clinical bacteria. GSC Adv Res Rev. 2021 Aug 30;8:078-84.
Kasra Kermanshahi R, Peymanfar S. Isolation and identification of Lactobacillus species with bacteriocin-like activity against MRSA. Iran J Basic Med Sci. 2020;23(9):1186-92.
Misganaw W, Teketay W. Isolation and identification of lactic acid bacteria from raw cow milk. Int J Adv Res Biol Sci. 2016;3(8):44-9.
Hamishehkar P, Shadmehr A, Mahmoodpoor S, Ozar M, Taher EM. Antimicrobial susceptibility patterns among bacteria isolated from intensive care units of the largest teaching hospital at the northwest of Iran. Iran J Microbiol. 2016;8(5):276-83.
Author E, Author F. Evaluating Antibacterial Activity of LAB Isolates Against Foodborne Pathogens Using Agar Overlay. Afr J Food Saf. 2025;
Eid R, Jakee JE, Rashidy A, Asfour H, Omara S, Kandil MM, et al. Potential antimicrobial activities of probiotic Lactobacillus strains isolated from raw milk. Probiotics Health. 2016;4:138.
Halder D, Mandal M, Chatterjee SS, Pal NK, Mandal S. Indigenous probiotic Lactobacillus isolates presenting antibiotic-like activity against human pathogenic bacteria. Biomedicines. 2017;5(4):31.
Tambekar DH, Bhutda SA. Studies on antimicrobial activity and characteristics of bacteriocins produced by Lactobacillus strains isolated from milk of domestic animals. Afr J Food Sci. 2010;4(4):181-6.
Silva CC, Silva SP, Ribeiro SC. Application of bacteriocins and protective cultures in dairy food preservation. Front Microbiol. 2018;9:594.
Espeche MC, Tomás MSJ, Wiese B, Bru E, Nader-Macías MEF. Physicochemical factors differentially affect the biomass and bacteriocin production by bovine Enterococcus mundtii CRL1656. J Dairy Sci. 2014;97(2):789-97.
Ibrahim F, Zafar B, Aman A, Ul Qader shah A, Ansari DA. Improvement of Lactobacillus plantarum for the enhanced production of bacteriocin like inhibitory substance using combinatorial approach. Biocatal Agric Biotechnol. 2019 Oct 1;22:101386.
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
