Optimisation of Acetylcholinesterase Extraction from the Brain of Clarias batrachus using Response Surface Methodology

Authors

  • M.A.F.S. Nazri Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • A.A. Basirun Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • M. Manogaran Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • S.A.M. Khalidi Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • M.K. Sabullah Fakulti Sains dan Sumber Alam, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
  • M.Y. Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.

DOI:

https://doi.org/10.54987/bstr.v7i2.485

Keywords:

Clarias batrachus; acetylcholinesterase; heavy metals; inhibitive assay

Abstract

Acetylcholinesterase is an important enzyme in biosensing works. It is utilized to detect insecticides and cheaper sources of the enzyme is constantly being sought. Optimisation of the acetylcholinesterase extraction from the brain of Clarias batrachus using Response Surface Methodology is reported for the first time in this study. AChE from the brain of this fish was extracted and partially purified using ammonium sulphate. The optimum range of ammonium sulphate cut was between 40 and 50%. RSM was conducted using Box-Behnken design and the optimisation value for the extraction was determined for each variable at the end of the study. The optimum concentrations of Triton X-100, PMSF, phosphate buffer and the pH for AChE extraction based on RSM were 0.75%, 1.00 mM, 0.075 M and 7.5, respectively.

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Published

2019-12-28

Issue

Vol. 7 No. 2 (2019)

Section

Articles

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How to Cite

Optimisation of Acetylcholinesterase Extraction from the Brain of Clarias batrachus using Response Surface Methodology. (2019). Bioremediation Science and Technology Research (e-ISSN 2289-5892), 7(2), 5-8. https://doi.org/10.54987/bstr.v7i2.485