Protein identification of vitellogenin in river catfish (Hemibagrus nemurus)

Authors

  • Roshani Othman Faculty of Science and Biotechnology, Universiti Selangor, Bestari Jaya Campus, Jalan Timur Tambahan, 45600 Batang Berjuntai, Selangor, Malaysia
  • Sharr Azni Harmin Faculty of Science and Biotechnology, Universiti Selangor, Bestari Jaya Campus, Jalan Timur Tambahan, 45600 Batang Berjuntai, Selangor, Malaysia
  • Ina-Salwany Md Yasin

DOI:

https://doi.org/10.54987/bstr.v3i1.244

Keywords:

Vitellogenin, Chromatography, Hemibagrus nemurus, River catfish, MALDI-TOF

Abstract

Mass production of fish broodstock with high quality eggs requires the knowledge on the chemical composition and physiochemical properties of vitellogenin (Vtg) during ovulation. Vtg is an egg yolk precursor phospholipoglycoprotein, and has been analysed to evaluate the reproductive conditions and determine the spawning period in captive and wild fish. In this study, Vtg was induced in male H. nemurus through three intramuscular injections of 17- estradiol (E2). The Vtg was purified from the serum using gel filtration chromatography and the
purified protein was reduced via SDS-PAGE. One major polypeptide corresponding to 130 kDa was observed. Vtg identification was done using peptide mass fingerprint (PMF) from the trypsin digestion of male H. nemurus Vtg induced with E2. The sequence homology of H. nemurus AYLAGAAADVLEVGVR matched the Vtg of other fish species when analysed
using MALDI-TOF. Vtg was confirmed by MASCOT at 95% significant level. The potential protein that controls the reproductive process and oocyte development isolated from this study was discussed to understand the structure and function of Vtg.

Downloads

Published

2015-11-02

Issue

Vol. 3 No. 1 (2015)

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

How to Cite

Protein identification of vitellogenin in river catfish (Hemibagrus nemurus). (2015). Bioremediation Science and Technology Research (e-ISSN 2289-5892), 3(1), 1-5. https://doi.org/10.54987/bstr.v3i1.244