The Effect of Substrate Concentration on Enzymatic Hydrolysis of Selected Food Waste for Glucose Production

Authors

  • Micky Vincent Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Sarawak, Malaysia.
  • Noor Amalina Mahamad Norrezi Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak
  • Kopli Bujang Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak
  • Dayang Salwani Awang Adeni Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, Kota Samarahan, 94300, Sarawak.

DOI:

https://doi.org/10.54987/bstr.v2i2.154

Keywords:

Glucose, Food waste Substrate concentration, Enzymatic hydrolysis, Glucose recovery

Abstract

Glucose is an important commodity for various domestic and industrial applications, ranging
from the manufacturing of food and pharmaceuticals to biofuel production. It is therefore
imperative to explore novel sources of glucose that are easy to process, cheap and
environmentally friendly. In this study, food wastes collected from restaurants and cafeteria
within Universiti Malaysia Sarawak (UNIMAS) campus were used as feedstock for glucose
production. Several feedstock loadings were investigated (7%, 10%, 20% and 30% w/v) in the
enzymatic hydrolysis processes that were carried out at pH 5.0, 100 rpm and 58 ± 2 °C for 24 h
using glucoamylase and crude protease preparations. Results showed that substrate
concentration of 20% (w/v) in batch hydrolysis experiments has the highest glucose production
at 140 g/L, with 46.20% glucose recovery. The highest amount of substrate being hydrolyzed
(68.35%) within 20 h was also recorded in the same experimental set. We conclude that
substrate concentration or feedstock loading during enzymatic hydrolysis is a very crucial
parameter for optimal glucose production and recovery from carbohydrate rich food wastes.

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Published

2015-01-13

Issue

Vol. 2 No. 2 (2014)

Section

Articles

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How to Cite

The Effect of Substrate Concentration on Enzymatic Hydrolysis of Selected Food Waste for Glucose Production. (2015). Bioremediation Science and Technology Research (e-ISSN 2289-5892), 2(2), 1-4. https://doi.org/10.54987/bstr.v2i2.154