The Effect of Malathion on The Activity of Cholinesterase From Freshwater Shrimp, Caridina Sp.

Authors

  • Mohd Khalizan . Sabullah Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.
  • Fisal Ahmad School of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.
  • Baskaran Gunasekaran Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • A.R.A. Rachman Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • Shi Yin Lee Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • Siti Aqlima Ahmad Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • M.Y. Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • Azlan Jualang Gansau Faculty of Science and Natural Resource, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.
  • Mohd Rosni Sulaiman Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.

DOI:

https://doi.org/10.54987/bessm.v2i2.193

Abstract

Acetylcholinesterase (AChE) from Caridina sp. was tested its sensitivity towards selected anticholinesterase such as malathion. Prior to this study, the optimal assay condition was determined at the temperature of 25oC, pH 8.0 and using phosphate buffer. At the concentration of 10-8 M, malathion was able to inhibit AChE activity at 1.75% and increased to 15.79% with the increasing of malathion concentration to 10-4 M. It is proven through this study that the crude extract of AChE is sensitive toward malathion exposure and provide an alternative study for biosensor development.

Published

2014-12-03

Issue

Vol. 2 No. 2 (2014)

Section

Articles

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How to Cite

The Effect of Malathion on The Activity of Cholinesterase From Freshwater Shrimp, Caridina Sp. (2014). Bulletin of Environmental Science and Sustainable Management (e-ISSN 2716-5353), 2(2), 36-38. https://doi.org/10.54987/bessm.v2i2.193